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Sigma
Extract-N-Amp™ Plant PCR Kit
sufficient for 1000 extractions, sufficient for 5000 amplifications
Description
| Components | Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix. |
| Other Notes | For additional information, please see www.sigma-aldrich.com/extract-n-amp. |
| Features and Benefits | • Single-step extraction of plant genomic DNA for PCR in less than 15 minutes • No freezing, mechanical disruption, organic extraction, column purification or precipitation required • Specially formulated PCR ReadyMix for use with extract • Hot Start antibody for highly specific PCR amplification of genomic DNA • REDExtract-N-Amp requires no loading buffers or tracking dyes required for gel analysis • Compatible with high-throughput requirements for genetic analysis of plants • Extract stable at 4 °C for at least 6 months |
| General description | The Extract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. A novel Extraction Solution eliminates the need for conventional freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column purification, or precipitation of DNA. |
| Legal Information | Extract-N-Amp is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
| Purchase of these products is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries. |
Properties
| usage | sufficient for 1000 extractions |
| sufficient for 5000 amplifications | |
| shipped in | wet ice |
| storage temp. | −20°C |
Safety
References
| Reference | Wang, D., et al., A High-Throughput System for the Rapid Extraction of Plant Genomic DNA for Genome Mapping and Marker-Assisted Breeding Studies J. Assoc. Lab. Autom. 10, 242, (2005) |
